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KMID : 0604520060320020075
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Journal of the Society of Cosmetic Scientists of Korea
2006 Volume.32 No. 2 p.75 ~ p.79
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Antioxidation and Inhibition of Matrix Metalloproteinase in UV-irradiated Human Dermal Fibroblast by Selaginella tamariscina
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Sim Gwan-Sub
Kim Jin-Hwa
Kim Jin-Hee
Lee Beom-Cheon
Pyo Hyeong-Bae
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Abstract
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In this study. we evaluated anti-aging activity of medical plants that protect the skin cell damage induced by UV irradiation. We have investigated diverse biological activities of Selaginella tamariscina as an anti-aging ingredient of cosmetics. S. tamariscina was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the IC_{50} values of 65.1{mu}g/mL against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 40.9 {mu}g/mL against superoxide radicals in the xanthine/xanthine oxidase system, respectively. For testing intracellular ROS scavenging activity, the cultured human dermal fibroblasts were analyzed by increase in dichlorofluorescein (DCF) fluorescence upon exposure to UVB 20 mJ/cm^2 after treatment of S. tamariscina. UVA-induced MMP-1 protein and mRNA expression in human dermal fibroblasts were reduced in a dose-dependent manner by S. tamariscina. Moreover, S. tamariscina inhibited MMP-2 (gelatinase) activity in UVA-irradiated human dermal fibroblasts assayed by zymography and semi-quantitative RT-PCR. Taken together, these results suggest that S. tamariscina may act as an anti-aging agent by Increasing collagen and preventing the skin cell damage induced by UV irradiation, and imply that S. tamariscina nay be useful as a new ingredient for anti-aging cosmetics.
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KEYWORD
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Selaginella tamariscina, anti-aging, ROS, antioxidant activity, MMP
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